BIOL 275 Laboratory Methods in Genomics • 6 Cr.
Introduces the use of laboratory tools and techniques to sequence DNA. Topics covered include DNA structures and gene expression. Emphasis on experimental methods and design. Students learn to think critically about research methodology and scientific investigation. Prerequisite: BIOL& 160 or BIOL& 211 or permission of instructor.
After completing this class, students should be able to:
- Describe the role of DNA science in genomics and biotechnology
- List structural, functional and organizational features of DNA and RNA
- Compare and contrast prokaryotic and eukaryotic gene structure function and regulation.
- Recognize the important features of genomic structure.
- Perform specific recombinant DNA techniques
- Demonstrate skill in storage, handling and quantification of DNA
- Describe methods used for DNA library construction and maintenance
- Demonstrate skill in DNA isolation and purification
- Compare and contrast DNA sequencing strategies
- Analyze DNA sequences using current software
- Use software required for construction and maintenance of genome database
- Demonstrate ability to present data in a scientific format
- Apply research strategies and scientific methods
- Discuss ethical, social and economic issues with genome science