CHEM 266 Biochemistry II • 5 Cr.
The second in a two-quarter sequence for science majors and for students interested in careers in pharmacy, dentistry, medicine, veterinary medicine and medical technology. Topics include lipid structure, metabolism, transport and biosynthesis, nucleic acid structure and function, DNA replication, transcription and translation. Prerequisite: CHEM 265
After completing this class, students should be able to:Lipid structure, transport and biological membranes • Describe the different types of lipids from both a chemical and a structural point of view • Explain the structure and properties of lipid bilayers and biological membranes • Describe digestion and absorption of triglycerides and lipid transport Lipid metabolism • Describe the stepwise process of fatty acid oxidation and fatty acid biosynthesis • Describe the pathway of cholesterol biosynthesis and cholesterol’s functions • Describe how fatty acids are stored and released in adipose tissue Amino acid metabolism • Describe the metabolic pathways resulting in glutamate, ammonia, and urea • Describe the different catabolic pathways of glucogenic and ketogenic amino acids • Define essential and non-essential amino acids and their biosynthesis Nucleotide biosynthesis • Explain the synthesis of purine and pyrimidine ribonucleotides and deoxyribonucleotides • Describe the different catabolic pathways of purines and pyrimidines Nucleic acid structure and function • Review the basic concepts in nucleic acid structure and function • Outline the basic techniques used in recombinant DNA technology • Compare and contrast different DNA and RNA structures DNA replication, repair and transcription • Describe the process of DNA replication in prokaryotes and eukaryotes • Identify the agents that can cause DNA damage and the different mechanisms used in DNA repair • Discuss transcription in prokaryotic and eukaryotic cells. Protein synthesis • Describe the organization of the genetic code and the mechanism of translation at the molecular level • Compare and contrast the structures of the prokaryotic and eukaryotic chromosomes • Explain the role of ubiquitin and the proteasome in protein degradation • Explain how proteins get transported through the Golgi stack The control of gene expression • Outline how gene expression is controlled in prokaryotes • Explain how chromatin structure influences gene expression in eukaryotes • Describe some of the posttranslational control mechanisms in eukaryotes