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CHEM 275 Introduction to Instrumental Analysis • 6 Cr.




Description starting Summer 2014

Designed for students interested in a career in molecular biosciences. Topics include measurements and calculations in analytical chemistry, interaction of radiation with matter, spectroscopy, mass spectrometry, and separation techniques. Format includes lecture, discussion, and laboratory. Prerequisite: CHEM& 163, and BIOL& 160 or BIOL& 211. Recommended: ENGL& 235 and BTS 147.


After completing this class, students should be able to:

  • Measurements in analytical chemistry
  • • Describe the nature and statistics of random and systematic errors
  • • Record, report, and present scientific data
  • • Choose appropriate equipment based on the sensitivity and precision required
  • Important calculations in analytical chemistry
  • • Solve stoichiometry problems
  • • Calculate concentrations and dilution factors in solution
  • • Calculate equilibrium constants in a variety of problems
  • Interaction of radiation with matter
  • • Describe the process of absorption of radiation by matter
  • • Explain how molecular absorption of radiation is related to electronic, vibrational, and rotational transitions
  • • Describe the process of emission of radiation by fluorescence and phosphorescence
  • Spectroscopy
  • • Apply Beer’s Law and explain its limitations
  • • Identify functional groups in organic molecules using infrared spectroscopy
  • • Operate different types of spectroscopy instrumentation
  • • Interpret spectroscopy data for qualitative and quantitative analysis
  • Mass Spectrometry
  • • Describe the basic principles by which mass spectrometry operates
  • • Identify the major components of a typical mass spectrometer
  • • Describe several common ionization methods
  • • Describe the advantages/disadvantages of different types of mass analyzers
  • • Interpret a mass spectrum for qualitative and quantitative analysis
  • Separation Techniques
  • • Describe the major principles of separation techniques
  • • Identify and describe the major components of various types of separation instrumentation
  • • Interpret chromatography data for qualitative and quantitative analysis
  • • Describe various chromatography techniques
  • • Describe other laboratory techniques used for separations, such as extraction, precipitation, centrifugation
  • • Choose and implement an appropriate separation technique for a practical application

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Contact info

Bellevue College
3000 Landerholm Circle SE Bellevue, WA 98007-6484 U.S.A.
Work: (425) 564-1000