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CHEM 275 Introduction to Instrumental Analysis • 6 Cr.

Department

Division

Description:

Designed for students interested in a career in molecular biosciences. Topics include measurements and calculations in analytical chemistry, interaction of radiation with matter, spectroscopy, mass spectrometry, and separation techniques. Format includes lecture, discussion, and laboratory. Prerequisite: CHEM& 163, and BIOL& 160 or BIOL& 211. Recommended: ENGL& 235 and BTS 147.

Outcomes:

After completing this class, students should be able to:

MEASURMENT IN ANALYTICAL CHEMISTRY
  • Describe the nature and statistics of random and systematic errors
  • Record, report, and present scientific data
  • Choose appropriate equipment based on the sensitivity and precision required
IMPORTANT CALCULATIONS IN ANALYTICAL CHEMISTRY
  • Solve stoichiometry problems
  • Calculate concentrations and dilution factors in solution
  • Calculate equilibrium constants in a variety of problems
INTERACTION OF RADIATION WITH MATTER
  • Describe the process of absorption of radiation by matter
  • Explain how molecular absorption of radiation is related to electronic, vibrational, and rotational transitions
  • Describe the process of emission of radiation by fluorescence and phosphorescence
SPECTROSCOPY
  • Apply Beer’s Law and explain its limitations
  • Identify functional groups in organic molecules using infrared spectroscopy
  • Operate different types of spectroscopy instrumentation
  • Interpret spectroscopy data for qualitative and quantitative analysis
MASS SPECTROMETRY
  • Describe the basic principles by which mass spectrometry operates
  • Identify the major components of a typical mass spectrometer
  • Describe several common ionization methods
  • Describe the advantages/disadvantages of different types of mass analyzers
  • Interpret a mass spectrum for qualitative and quantitative analysis
SEPARATION TECHNIQUES
  • Describe the major principles of separation techniques
  • Identify and describe the major components of various types of separation instrumentation
  • Interpret chromatography data for qualitative and quantitative analysis
  • Describe various chromatography techniques
  • Describe other laboratory techniques used for separations, such as extraction, precipitation, centrifugation
  • Choose and implement an appropriate separation technique for a practical application
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Bellevue College
3000 Landerholm Circle SE Bellevue, WA 98007-6484 U.S.A.
Work: (425) 564-1000